Excepting for the burramyids and the monotypic Hypsiprymnodon, this alignment includes representatives from all diprotodontian families: Phascolarctos cinereus (Koala, NC_008133), Vombatus ursinus (common wombat, NC_003322), Marcropus robustus (wallaroo, NC_001794), Potorous longipes (long-nosed potoroo, NC_006524), Lagorchestes hirsutus (rufous hare-wallaby, NC_008136), Trichosurus vulpecula (common brushtail possum, NC_003039), Phalanger interpositus (Stein's cuscus, NC_008137), Distoechurus pennatus (feather-tailed possum, NC_008145), Tarsipes rostratus (honey possum, NC_006518), Pseudocheirus peregrinus (common ringtail possum, NC_006519), Dactylopsila trivirgata (striped possum, NC_008134) and Petaurus sp. The primary dataset includes complete mt genome protein, rRNA and tRNA coding sequences, totaling 14,645 nucleotides after alignment in Se-Al 2.0a9 ( Rambaut, 1996) and exclusion of apporoximately 690 nucleotide sites with ambiguous homology (mostly from RNA loop regions). "It is a useful aid to choosing software, the material on the design of an introductory course will be useful to some, and parts of it will serve as good guides to particular pieces of software. "This book gives many details concerning the computational challenges facing biologists and their likely future solutions, cities shareware that can be used to build an analysis suite to meet specific needs, describes software, databases, and other resources available on the www, discusses common image analysis techniques, reviews oligonucleotide and PCR-primer design, serves as an important bioinformatics teaching resource and provides, a tested course design and resource for an introductory graduate level course." - Cellular and Molecular Biology
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It is a solid introduction to the practical aspects of bioinformatics research." -Biotech Software & Internet Report
Bioinformatics Methods and Protocols is an invaluable resource for this target audience.
The focus on inexpensive tools and resources is also likely to be very popular with those scientists new to the field and possibly just starting their careers as independent researchers. "This book is an interesting mix of the practical and theoretical aspects of bioinformatics, and those readers interested in obtaining information on specific tools and resources will not be disappointed.this book provides a nice overview of a number of different aspects of the field. ".there are some real gems in Bioinformatics: Methods and Protocols the chapter on phylogenetic analysis presents enough information to produce a tree that one could submit for publication without too much apprehension, and William Pearson's clear and trenchant discussion of his FASTA programs (and the similar BLAST suite) alone might justify the purchase of this book." -Tibtech
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It is remarkably complete and could be extremely useful for those laboratories that are thinking seriously about their bioinformatic requirements.The chapters are clearly laid out and the text is generally easy to read and refreshingly free of much of the jargon that can be found in bioinformatics.I would highly recommend it for institutional purchase." -Microbiology Today "There has been a need for a book of this kind for some time. It constitutes a gold-standard reference for today's scientists who wish to develop and hone their bioinformatics skills towards the discovery of new biological relationships.
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The book also includes a set of guidelines for designing and teaching an introductory bioinformatics course and numerous illustrative examples to teach the reader how to solve problems.īioinformatics: Methods and Protocols offers to experienced and novice biologists a broad overview of the computational tools that have reshaped modern biology. Web-based resources are examined for primary sequence databases, primary sequence analysis methods, and clinical databases useful in molecular medicine. Molecular biology software includes Genotator and sequence-similarity searching using FASTA, CLUSTAL multiple sequence alignment, and phylogenic analysis. Among the sequence analysis systems reviewed are GCG, Omiga, MacVector, DNASTAR, PepTool, GeneTool, and Staden. In Bioinformatics: Methods and Protocols, hands-on users and experts survey the key biological software packages, offering useful tips and an overview of current developments.
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